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\nPerkins Est 2011b Keygen Free download! Best Software, Games, Softwares and Laptop/Desktop Software .An exonuclease gene of herpes simplex virus type 1, tj110: its restriction map and phylogenetic analysis.\nThe location and function of a gene encoding a herpes simplex virus (HSV) exonuclease, named tj110, were determined by constructing a restriction map of HindIII fragments of the DNA of HSV type 1 strain (V7189). The gene mapped between BamHI and XhoI fragments at coordinates 73.2 min and 89.1 min in the DNA of virus strain 18. The gene was located between the BamHI and XhoI cleavage sites in a 1734-bp stretch of DNA which coded for a 377 amino acid protein. Analysis of the amino acid sequence of the protein indicated two regions (amino acid residues 47 to 81 and 168 to 192) with a moderate level of sequence homology to other enzymes of a similar family, the tRNA splicing endonucleases. The tj110 gene was mapped to an intermediate site between loci tn and dnaQ in the HSV-1 genome. Recombination between tj110 and dnaQ was confirmed by the data of a recombination experiment performed by transformation of Escherichia coli JM107 with dnaQ- and tj110-containing DNA isolated from an HSV-1-B mutant. Comparison of the tj110 amino acid sequence with that of the catalytic subunit of the E. coli exonuclease II (Exo II) showed a distant homology in one of the domains, the catalytic core, which is composed of five histidine residues. The same homology was found when the amino acid sequence of tj110 was aligned to the amino acid sequence of Exo II. The homology of the remaining domains of tj110 and Exo II was low, supporting the idea that tj110 and Exo II probably evolved from a common enzyme ancestor and diverged after the separation of the two cells. U.S.C. § 1202(c)(2); and
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