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In particular, glucose is necessary for the maintenance of a patient during hypoglycemic therapy and continuous monitoring of glucose levels is a critical care measure in the prevention of future diabetic complications.\nCommercially available methods for measuring glucose levels require the extraction of the blood sample and subsequent chemical conversion of the resulting glucose. Drawbacks to these methods include the contamination of the sample by substances extraneous to the blood and the necessity for frequent sampling. A major need exists for a non-extraction method which requires no equipment, no special sampling techniques or instruments, and provides results with sufficient rapidity to be of diagnostic value.\nVarious chemical reactions have been suggested which can convert glucose to a chromophore which can be detected spectrophotometrically. European Patent No. 0.039.944 describes the generation of the chromophore by the interaction of glucose with a polycarboxylic acid. European Patent No. 0.086.430 provides a method for obtaining 3,4,5,6-tetra-O-acetyl-D-glucose as a chromophore.\nIn U.S. Pat. No. 4,668,520, a method for the measurement of glucose by the interaction of glucose with a diazidic compound is described.\nThese reactions require the use of an acidic medium to effect chromophore formation. The pH's needed to generate chromophore are in the range of 4.6 to 6.0 which limits the types of chromogenic compounds that can be used.\nIt would be desirable to have a non-extraction method which produces a readily measurable color whose intensity is proportional to the concentration of glucose. 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